DOI: 10.22184/1993-8578.2021.14.7-8.424.428

The optical microscope is one of the most common and simple tools for studying objects on the microscale, especially in the biological sciences, biomedicine, and chemistry. A significant limitation for use of the optical microscopy is the optical diffraction limit, which is about 250 nm in white light at a wavelength of about 550 nm and a microscope numerical aperture of 1.0. This resolution is often insufficient for imaging tasks of cell organelles, tissue, viral and bacterial particles. A microsphere placed on the sample surface can overcome this limitation and visualise structures as small as 25 nm.


Разработка: студия Green Art