To date, the mechanism responsible for photobiomodulation oxidative stress induced by 1265 nm laser light is unclear, but mitochondria are considered the most probable acceptors of laser radiation at this wavelength. We studied oxidative stress, mitochondrial potential, the level of reduced glutathione, and the viability of human adenocarcinoma cells. Narrow-band and broad-band laser irradiation at the doses of 9.45 and 66.6-400 J/cm2, respectively, caused cell death, an increase of reactive oxygen species intracellular concentration and a disturbance of the mitochondrial functions. Thus, lasers at the wavelength of 1265 nm affect HCT116 cells through mitochondrial damage, and the dose increase contributes to cell damage without the heating effect.


Разработка: студия Green Art