DOI: https://doi.org/10.22184/1993-8578.2024.17.7-8.418.425

Controlled manipulation of cultured cells and local delivery of macromolecules and substances are still unsolved problems in experimental biology. Intracellular injection of various therapeutic agents, including biologics and supramolecular agents, is difficult due to natural biological barriers required to protect the cell. Efficient delivery of nucleic acids, proteins, peptides and nanoparticles is critical for clinical implementation of new technologies that can benefit the treatment of diseases using gene and cell therapy. Using a capillary, it is possible to locally apply a desired substance to a cell or even introduce it into the cell, and then evaluate its effect on morphology using scanning capillary microscopy (SCM) tools. These capabilities make the capillary microscopy method promising for biomedical purposes.