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Issue #7-8/2024
K.E.Mochalov, O.I.Agapova, I.I.Agapov, D.S.Korzhov, D.O.Solovyeva, S.V.Sizova, M.S.Shestopalova, V.A.Oleinikov, A.E.Efimov
FLUORESCENCE OPTICAL NANOTOMOGRAPHY: COMBINING THE TECHNIQUES OF TOTAL INTERNAL REFLECTION FLUORESCENCE MICROSCOPY AND ULTRAMICROTOMY
FLUORESCENCE OPTICAL NANOTOMOGRAPHY: COMBINING THE TECHNIQUES OF TOTAL INTERNAL REFLECTION FLUORESCENCE MICROSCOPY AND ULTRAMICROTOMY
DOI: https://doi.org/10.22184/1993-8578.2024.17.7-8.428.433
A method of 3D-TIRF microscopy is proposed, based on combination of ultramicrotomy and total internal reflection fluorescence microscopy techniques of the sample surface after cutting, which allows reconstructing the three-dimensional ultrastructure of objects.
A method of 3D-TIRF microscopy is proposed, based on combination of ultramicrotomy and total internal reflection fluorescence microscopy techniques of the sample surface after cutting, which allows reconstructing the three-dimensional ultrastructure of objects.
Теги: 3d-tirf microscopy 3d-tirf-микроскопия three-dimensional ultrastructure of objects трехмерная ультраструктура объектов
Received: 17.10.2024 | Accepted: 21.10.2024 | DOI: https://doi.org/10.22184/1993-8578.2024.17.7-8.428.433
Original paper
FLUORESCENCE OPTICAL NANOTOMOGRAPHY:
COMBINING THE TECHNIQUES OF TOTAL INTERNAL REFLECTION FLUORESCENCE MICROSCOPY AND ULTRAMICROTOMY
K.E.Mochalov1, Doct. of Sci. (Physics and Mathematics), Senior Researcher, ORCID: 0000-0001-8157-9613
O.I.Agapova2, Cand. of Sci. (Biology), Senior Researcher, ORCID: 0000-0002-4507-1852
I.I.Agapov2, Doct. of Sci. (Biology), Prof., Head of Laboratory, ORCID: 0000-0002-0273-4601
D.S.Korzhov1, 3, Technician, ORCID: 0009-0006-2626-1278
D.O.Solovyeva1, Cand. of Sci. (Biology), Researcher, ORCID: 0000-0002-2590-4204
S.V.Sizova1, Cand. of Sci. (Chemistry), Researcher, ORCID: 0000-0003-0846-4670
M.S.Shestopalova1, 3, Engineer Researcher, ORCID: 0000-0001-6543-4289
V.A.Oleinikov1, 3, Doct. of Sci. (Physics and Mathematics),
Prof., Head of Department, ORCID: 0000-0003-4623-4913
A.E.Efimov2, Doct. of Sci. (Biology), Chief Researcher, ORCID: 0000-0002-0769-301X / antefimov@gmail.com
Abstract. A method of 3D-TIRF microscopy is proposed, based on combination of ultramicrotomy and total internal reflection fluorescence microscopy techniques of the sample surface after cutting, which allows reconstructing the three-dimensional ultrastructure of objects.
Keywords: 3D-TIRF microscopy, three-dimensional ultrastructure of objects
For citation: K.E. Mochalov, O.I. Agapova, I.I. Agapov, D.S. Korzhov, D.O. Solovyeva, S.V. Sizova, M.S. Shestopalova, V.A. Oleinikov, A.E. Efimov. Fluorescence optical nanotomography: combining the techniques of total internal reflection fluorescence microscopy and ultramicrotomy. NANOINDUSTRY. 2024. Vol. 17. No. 7–8. PP. 428–433. https://doi.org/10.22184/1993-8578.2024.17.7-8.428.433.
Original paper
FLUORESCENCE OPTICAL NANOTOMOGRAPHY:
COMBINING THE TECHNIQUES OF TOTAL INTERNAL REFLECTION FLUORESCENCE MICROSCOPY AND ULTRAMICROTOMY
K.E.Mochalov1, Doct. of Sci. (Physics and Mathematics), Senior Researcher, ORCID: 0000-0001-8157-9613
O.I.Agapova2, Cand. of Sci. (Biology), Senior Researcher, ORCID: 0000-0002-4507-1852
I.I.Agapov2, Doct. of Sci. (Biology), Prof., Head of Laboratory, ORCID: 0000-0002-0273-4601
D.S.Korzhov1, 3, Technician, ORCID: 0009-0006-2626-1278
D.O.Solovyeva1, Cand. of Sci. (Biology), Researcher, ORCID: 0000-0002-2590-4204
S.V.Sizova1, Cand. of Sci. (Chemistry), Researcher, ORCID: 0000-0003-0846-4670
M.S.Shestopalova1, 3, Engineer Researcher, ORCID: 0000-0001-6543-4289
V.A.Oleinikov1, 3, Doct. of Sci. (Physics and Mathematics),
Prof., Head of Department, ORCID: 0000-0003-4623-4913
A.E.Efimov2, Doct. of Sci. (Biology), Chief Researcher, ORCID: 0000-0002-0769-301X / antefimov@gmail.com
Abstract. A method of 3D-TIRF microscopy is proposed, based on combination of ultramicrotomy and total internal reflection fluorescence microscopy techniques of the sample surface after cutting, which allows reconstructing the three-dimensional ultrastructure of objects.
Keywords: 3D-TIRF microscopy, three-dimensional ultrastructure of objects
For citation: K.E. Mochalov, O.I. Agapova, I.I. Agapov, D.S. Korzhov, D.O. Solovyeva, S.V. Sizova, M.S. Shestopalova, V.A. Oleinikov, A.E. Efimov. Fluorescence optical nanotomography: combining the techniques of total internal reflection fluorescence microscopy and ultramicrotomy. NANOINDUSTRY. 2024. Vol. 17. No. 7–8. PP. 428–433. https://doi.org/10.22184/1993-8578.2024.17.7-8.428.433.
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