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Issue #7-8/2024
A.V.Moiseenko, N.A.Basalova, D.V.Bagrov, T.S.Trifonova, M.A.Vigovsky, U.D.Dyachkova, O.A.Grigorieva, E.S.Novoseletskaya, A.Yu.Efimenko, O.S.Sokolova
DIRECT VISUALIZATION OF EXTRACELLULAR VESICLES ON THE MEMBRANE OF HUMAN MESENCHYMAL STEM/STROMAL CELLS BY CRYO-ELECTRON MICROSCOPY
DIRECT VISUALIZATION OF EXTRACELLULAR VESICLES ON THE MEMBRANE OF HUMAN MESENCHYMAL STEM/STROMAL CELLS BY CRYO-ELECTRON MICROSCOPY
| DOI: https://doi.org/10.22184/1993-8578.2024.17.7-8.434.443
Extracellular vesicles (EVs) play an important role in intercellular communication and influence a wide range of physiological and pathological processes. Membrane-associated extracellular vesicles (MAVs) represent a distinct and poorly understood class of EVs. This study demonstrates the application of cryo-electron microscopy (cryo-EM) to investigate MAVs secreted by human mesenchymal stem/stromal cells (MSCs). Cryo-EM revealed vesicles ranging in diameter from 50 to 750 nm located near the cell surface. The results obtained will facilitate further studies on the physiological role of MAVs and their association with cell membranes.
Tags: cryo-electron microscopy extracellular vesicles membrane-associated vesicles stem cells внеклеточные везикулы криоэлектронная микроскопия мембранно-ассоциированные везикулы стволовые клетки
Subscribe to the journal Nanoindustry to read the full article.
Extracellular vesicles (EVs) play an important role in intercellular communication and influence a wide range of physiological and pathological processes. Membrane-associated extracellular vesicles (MAVs) represent a distinct and poorly understood class of EVs. This study demonstrates the application of cryo-electron microscopy (cryo-EM) to investigate MAVs secreted by human mesenchymal stem/stromal cells (MSCs). Cryo-EM revealed vesicles ranging in diameter from 50 to 750 nm located near the cell surface. The results obtained will facilitate further studies on the physiological role of MAVs and their association with cell membranes.
Tags: cryo-electron microscopy extracellular vesicles membrane-associated vesicles stem cells внеклеточные везикулы криоэлектронная микроскопия мембранно-ассоциированные везикулы стволовые клетки
Subscribe to the journal Nanoindustry to read the full article.
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