rus
Issue #3-4/2025
Yu.D.Ivanov, I.D.Shumov, E.E.Vazhenkova, A.N.Ableev, A.F.Kozlov, A.V.Vinogradova, E.D.Nevedrova, O.N.Afonin, V.Yu.Tatur, A.A.Lukyanitsa, A.L.Shishkin, N.D.Ivanova, D.V.Enikeev, E.S.Yushkov, М.М. Kuznetsov, A.Yu. Dolgoborodov, V.S.Ziborov
THE EFFECT OF CAVITATED WATER ON THE PHYSICOCHEMICAL PROPERTIES OF HORSERADISH PEROXIDASE AS STUDIED AT THE LEVEL OF SINGLE ENZYME MOLECULES. TIME DEPENDENCE OF CAVITATION EFFECT ON ENZYME PROPERTIES
THE EFFECT OF CAVITATED WATER ON THE PHYSICOCHEMICAL PROPERTIES OF HORSERADISH PEROXIDASE AS STUDIED AT THE LEVEL OF SINGLE ENZYME MOLECULES. TIME DEPENDENCE OF CAVITATION EFFECT ON ENZYME PROPERTIES
DOI: https://doi.org/10.22184/1993-8578.2025.18.3-4.194.202
The effect of water subjected to cavitation and kept after cavitation for 8 months on the enzyme horseradish peroxidase (HRP) was studied using atomic force microscopy (AFM) and spectrophotometry (SP). No significant changes in the adsorption of HRP on freshly cleaved mica were detected compared to the control enzyme sample. On the contrary, the enzymatic activity of HRP after incubation in water subjected to cavitation 8 months ago decreased twofold. After keeping the enzyme solution in water subjected to cavitation, compared to the control enzyme sample. The detected effect should be considered in the development of biotechnological processes that involve the use of liquid media cavitation.
Tags: cavitation enzyme adsorption horseradish peroxidase адсорбция фермента кавитация пероксидаза хрена
Subscribe to the journal Nanoindustry to read the full article.
The effect of water subjected to cavitation and kept after cavitation for 8 months on the enzyme horseradish peroxidase (HRP) was studied using atomic force microscopy (AFM) and spectrophotometry (SP). No significant changes in the adsorption of HRP on freshly cleaved mica were detected compared to the control enzyme sample. On the contrary, the enzymatic activity of HRP after incubation in water subjected to cavitation 8 months ago decreased twofold. After keeping the enzyme solution in water subjected to cavitation, compared to the control enzyme sample. The detected effect should be considered in the development of biotechnological processes that involve the use of liquid media cavitation.
Tags: cavitation enzyme adsorption horseradish peroxidase адсорбция фермента кавитация пероксидаза хрена
Subscribe to the journal Nanoindustry to read the full article.
Readers feedback
