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Issue #3-4/2025
V.S.Ziborov, I.D.Shumov, E.E.Vazhenkova, A.N.Ableev, A.F.Kozlov, A.V.Vinogradova, E.D.Nevedrova, O.N.Afonin, V.Yu.Tatur, A.A.Lukyanitsa, A.V.Shcherbakov, N.D.Ivanova, E.S.Yushkov, D.V.Enikeev, Yu.D.Ivanov
THE EFFECT OF CAVITED WATER ON PHYSICOCHEMICAL PROPERTIES OF HORSERADISH PEROXIDASE AS STUDIED AT THE LEVEL OF SINGLE ENZYME MOLECULES. CHANGE OF ENZYME PROPERTIES UNDER THE INFLUENCE OF CAVITATION
THE EFFECT OF CAVITED WATER ON PHYSICOCHEMICAL PROPERTIES OF HORSERADISH PEROXIDASE AS STUDIED AT THE LEVEL OF SINGLE ENZYME MOLECULES. CHANGE OF ENZYME PROPERTIES UNDER THE INFLUENCE OF CAVITATION
DOI: https://doi.org/10.22184/1993-8578.2025.18.3-4.184.192
The effect of water subjected to cavitation on horseradish peroxidase (HRP) enzyme is studied using atomic force microscopy (AFM) and spectrophotometry (SP). By AFM, a significant change in the adsorption of HRP on freshly cleaved mica after keeping the enzyme solution in water subjected to cavitation, is found – as compared to the control enzyme sample. According to SP, the enzymatic activity of HRP does not change. The effect observed is useful to take into account when working with enzymes on industrial and research equipment, in which cavitation may occur in the flow of an aqueous medium.
Tags: cavitation enzyme adsorption horseradish peroxidase адсорбция фермента кавитация пероксидаза хрена
Subscribe to the journal Nanoindustry to read the full article.
The effect of water subjected to cavitation on horseradish peroxidase (HRP) enzyme is studied using atomic force microscopy (AFM) and spectrophotometry (SP). By AFM, a significant change in the adsorption of HRP on freshly cleaved mica after keeping the enzyme solution in water subjected to cavitation, is found – as compared to the control enzyme sample. According to SP, the enzymatic activity of HRP does not change. The effect observed is useful to take into account when working with enzymes on industrial and research equipment, in which cavitation may occur in the flow of an aqueous medium.
Tags: cavitation enzyme adsorption horseradish peroxidase адсорбция фермента кавитация пероксидаза хрена
Subscribe to the journal Nanoindustry to read the full article.
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