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Issue #1/2026
I.D.Shumov, V.S.Ziborov, A.F.Kozlov, E.E.Vazhenkova, A.N.Ableev, A.V.Vinogradova, E.D.Nevedrova, O.B.Kovalev, A.L.Rossina, D.V.Enikeev, N.V.Burundaeva, V.Yu.Tatur, A.A.Lukyanitsa, N.D.Ivanova, E.S.Yushkov, O.N.Afonin, Yu.D.Ivanov
INFLUENCE OF INCUBATION OF HORSERADISH PEROXIDASE SOLUTION ON THE HUMAN BODY ON THE ADSORPTION PROPERTIES AND ACTIVITY OF THE ENZYME: DIFFERENCES IN NORMAL STATE AND IN CARDIOVASCULAR PATHOLOGY
INFLUENCE OF INCUBATION OF HORSERADISH PEROXIDASE SOLUTION ON THE HUMAN BODY ON THE ADSORPTION PROPERTIES AND ACTIVITY OF THE ENZYME: DIFFERENCES IN NORMAL STATE AND IN CARDIOVASCULAR PATHOLOGY
DOI: https://doi.org/10.22184/1993-8578.2026.19.1.20.31
By atomic force microscopy (AFM) and spectrophotometry (SP), the effect of incubating a horseradish peroxidase (HRP) solution near the wrist of a patient with cardiovascular disease (CVD), as well as near the wrist of a healthy volunteer, on the adsorption properties and activity of this enzyme has been studied. After the incubation, adsorption of HRP aggregates onto freshly cleaved mica, along with the monomeric form of the enzyme, was observed near the wrist of the patient with CVD. No adsorption of HRP aggregates was observed in the case of the healthy volunteer, nor with the control enzyme sample. However, the enzymatic activity of HRP relative to the control sample increased in both cases. Given the wide use of HRP in medical diagnostics and the importance of peroxidases as CVD biomarkers, these experimental findings may be useful in developing new diagnostic systems for human diseases, including CVD and inflammatory conditions.
Subscribe to the journal Nanoindustry to read the full article.
By atomic force microscopy (AFM) and spectrophotometry (SP), the effect of incubating a horseradish peroxidase (HRP) solution near the wrist of a patient with cardiovascular disease (CVD), as well as near the wrist of a healthy volunteer, on the adsorption properties and activity of this enzyme has been studied. After the incubation, adsorption of HRP aggregates onto freshly cleaved mica, along with the monomeric form of the enzyme, was observed near the wrist of the patient with CVD. No adsorption of HRP aggregates was observed in the case of the healthy volunteer, nor with the control enzyme sample. However, the enzymatic activity of HRP relative to the control sample increased in both cases. Given the wide use of HRP in medical diagnostics and the importance of peroxidases as CVD biomarkers, these experimental findings may be useful in developing new diagnostic systems for human diseases, including CVD and inflammatory conditions.
Subscribe to the journal Nanoindustry to read the full article.
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